Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues: Evaluation of extraction methodologies and characterization of value-added bioactive compounds (2024)

Durán-Aranguren, D. D., Villabona, L. C., López, G. D., Carazzone, C., Sierra, R., Posada, J. A., & Mussatto, S. I. (2025). Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues: Evaluation of extraction methodologies and characterization of value-added bioactive compounds. Separation and Purification Technology, 359, Article 130590. https://doi.org/10.1016/j.seppur.2024.130590

Durán-Aranguren, D. D. ; Villabona, L. C. ; López, G. D. et al. / Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues : Evaluation of extraction methodologies and characterization of value-added bioactive compounds. In: Separation and Purification Technology. 2025 ; Vol. 359.

@article{e0ae993eb7b9476ba553d51e73886b2a,

title = "Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues: Evaluation of extraction methodologies and characterization of value-added bioactive compounds",

abstract = "Residues from orange processing are being continuously generated in vast amounts due to the increasing demand for this fruit and its byproducts worldwide. The valorization of Orange Residues is challenging in contrast to conventional “lignocellulosic residues” since this fruit-derived biomass contains high amounts of pectin and an extractive fraction rich in sugars, essential oils, and polyphenols. The relative amounts of these fractions are highly influenced by the juice/pulp extraction process. Even though several studies have explored how to produce added value from this biomass, it is necessary to compare how different techniques and operating conditions influence the bioactive compounds that can be recovered and the remnant biomass after processing. This study compares essential oil extraction, solvent extraction, and acid hydrolysis for fermentable sugar and pectin production to elucidate a feasible sequence for a biorefinery from Orange Residues. From our results, it was proposed a technically feasible sequence that maximizes the yields of i) essential oils (0.70 ± 0.05 g/ 100 g DM) from steam distillation (4 h, 1500 W), ii) naringin (0.19 g/100 g DM), hesperidin (1.27 g/100 g DM), and glucose (3.9 g/100 g DM) from solid-liquid extraction (Ethanol 61.6 % (w/v), 45.8 °C, 155.5 min, and 5 % (w/v) biomass load), iii) pectin (25.24 g/100 g DM) from citric acid hydrolysis (pH 1.5, 90 °C, 82.1 min, and 5 % (w/v) biomass load), and iv) glucose (12.41 g/100 g DM) and xylose (10.13 g/100 g DM) from sulfuric acid hydrolysis (Sulfuric acid 0.68 % (w/v), 121 °C, 24.1 min, and 7.32 % (w/v) biomass load), in a biorefinery scheme.",

keywords = "Bioactive compounds, Biomass valorization, Biorefinery, Orange residues, Process conditions",

author = "Dur{\'a}n-Aranguren, {D. D.} and Villabona, {L. C.} and L{\'o}pez, {G. D.} and C. Carazzone and R. Sierra and Posada, {J. A.} and Mussatto, {S. I.}",

year = "2025",

doi = "10.1016/j.seppur.2024.130590",

language = "English",

volume = "359",

journal = "Separation and Purification Technology",

issn = "1383-5866",

publisher = "Elsevier",

}

Durán-Aranguren, DD, Villabona, LC, López, GD, Carazzone, C, Sierra, R, Posada, JA & Mussatto, SI 2025, 'Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues: Evaluation of extraction methodologies and characterization of value-added bioactive compounds', Separation and Purification Technology, vol. 359, 130590. https://doi.org/10.1016/j.seppur.2024.130590

Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues: Evaluation of extraction methodologies and characterization of value-added bioactive compounds. / Durán-Aranguren, D. D.; Villabona, L. C.; López, G. D. et al.
In: Separation and Purification Technology, Vol. 359, 130590, 2025.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues

T2 - Evaluation of extraction methodologies and characterization of value-added bioactive compounds

AU - Durán-Aranguren, D. D.

AU - Villabona, L. C.

AU - López, G. D.

AU - Carazzone, C.

AU - Sierra, R.

AU - Posada, J. A.

AU - Mussatto, S. I.

PY - 2025

Y1 - 2025

N2 - Residues from orange processing are being continuously generated in vast amounts due to the increasing demand for this fruit and its byproducts worldwide. The valorization of Orange Residues is challenging in contrast to conventional “lignocellulosic residues” since this fruit-derived biomass contains high amounts of pectin and an extractive fraction rich in sugars, essential oils, and polyphenols. The relative amounts of these fractions are highly influenced by the juice/pulp extraction process. Even though several studies have explored how to produce added value from this biomass, it is necessary to compare how different techniques and operating conditions influence the bioactive compounds that can be recovered and the remnant biomass after processing. This study compares essential oil extraction, solvent extraction, and acid hydrolysis for fermentable sugar and pectin production to elucidate a feasible sequence for a biorefinery from Orange Residues. From our results, it was proposed a technically feasible sequence that maximizes the yields of i) essential oils (0.70 ± 0.05 g/ 100 g DM) from steam distillation (4 h, 1500 W), ii) naringin (0.19 g/100 g DM), hesperidin (1.27 g/100 g DM), and glucose (3.9 g/100 g DM) from solid-liquid extraction (Ethanol 61.6 % (w/v), 45.8 °C, 155.5 min, and 5 % (w/v) biomass load), iii) pectin (25.24 g/100 g DM) from citric acid hydrolysis (pH 1.5, 90 °C, 82.1 min, and 5 % (w/v) biomass load), and iv) glucose (12.41 g/100 g DM) and xylose (10.13 g/100 g DM) from sulfuric acid hydrolysis (Sulfuric acid 0.68 % (w/v), 121 °C, 24.1 min, and 7.32 % (w/v) biomass load), in a biorefinery scheme.

AB - Residues from orange processing are being continuously generated in vast amounts due to the increasing demand for this fruit and its byproducts worldwide. The valorization of Orange Residues is challenging in contrast to conventional “lignocellulosic residues” since this fruit-derived biomass contains high amounts of pectin and an extractive fraction rich in sugars, essential oils, and polyphenols. The relative amounts of these fractions are highly influenced by the juice/pulp extraction process. Even though several studies have explored how to produce added value from this biomass, it is necessary to compare how different techniques and operating conditions influence the bioactive compounds that can be recovered and the remnant biomass after processing. This study compares essential oil extraction, solvent extraction, and acid hydrolysis for fermentable sugar and pectin production to elucidate a feasible sequence for a biorefinery from Orange Residues. From our results, it was proposed a technically feasible sequence that maximizes the yields of i) essential oils (0.70 ± 0.05 g/ 100 g DM) from steam distillation (4 h, 1500 W), ii) naringin (0.19 g/100 g DM), hesperidin (1.27 g/100 g DM), and glucose (3.9 g/100 g DM) from solid-liquid extraction (Ethanol 61.6 % (w/v), 45.8 °C, 155.5 min, and 5 % (w/v) biomass load), iii) pectin (25.24 g/100 g DM) from citric acid hydrolysis (pH 1.5, 90 °C, 82.1 min, and 5 % (w/v) biomass load), and iv) glucose (12.41 g/100 g DM) and xylose (10.13 g/100 g DM) from sulfuric acid hydrolysis (Sulfuric acid 0.68 % (w/v), 121 °C, 24.1 min, and 7.32 % (w/v) biomass load), in a biorefinery scheme.

KW - Bioactive compounds

KW - Biomass valorization

KW - Biorefinery

KW - Orange residues

KW - Process conditions

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U2 - 10.1016/j.seppur.2024.130590

DO - 10.1016/j.seppur.2024.130590

M3 - Article

AN - SCOPUS:85209376491

SN - 1383-5866

VL - 359

JO - Separation and Purification Technology

JF - Separation and Purification Technology

M1 - 130590

ER -

Durán-Aranguren DD, Villabona LC, López GD, Carazzone C, Sierra R, Posada JA et al. Recovery of essential oils, polyphenols, fermentable sugars, and pectin from orange residues: Evaluation of extraction methodologies and characterization of value-added bioactive compounds. Separation and Purification Technology. 2025;359:130590. doi: 10.1016/j.seppur.2024.130590